Pdf isolation of biosurfactant producing bacteria from petroleum. Isolation of crude oil degrading marine bacteria and. In this research six different genus of biosurfactant producing bacteria were isolated from persian gulf at bushehr provenance. Isolation and characterization of biosurfactant producing bacteria from oil contaminated soil a thesis submitted to the national institute of technology, rourkela in partial fulfillment of the requirements of the degree of bachelor of technology chemical engineering by ajeet kumar soni roll no. After screening, candidate biosurfactantproducing bacteria were selected for optimization of the physical and. Screening and isolation of biosurfactant producing bacteria from. Screening and isolation of biosurfactant producing.
Isolation of biosurfactant producing bacteria from oil. Original article characterization and phylogenetic analysis of biosurfactantproducing bacteria isolated from palm oil contaminated soils kanokrat saisaard1, atipan saimmai2,3, and suppasil maneerat4 1 faculty of science and technology, suratthani rajabhat university, mueang, surat thani, 84100 thailand. Pruthi and cameotra 1997 screened biosurfactant producing bacteria by assay the cell surface hydrophobicity. The use of selective media containing sucrose 10 g. Wide biosurfactant application on biorremediation is limited by its high production cost. B screening and isolation of biosurfactantproducing bacteria bacteria were isolated from above samples as follows. Among the various bioactive compounds, biosurfactants bioemulsifiers are attracting major interest and. Twenty three morphologically distinct microbial colonies 14 gram positive including one coccus and bacilli.
Enhanced biosurfactant production was carried out using olive oil as the carbon. Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. The word surfactant is derived from the term surface active agent. Antibiotic sensitivity test for biosurfactant producing bacteria 8. Biosurfactant have special advantage over their commercially manufactured counterparts because of its low toxicity, biodegradable nature, and effectiveness at extreme temperature, ph, salinity and ease of synthesis 9. Isolation of crude oil degrading marine bacteria and assessment for biosurfactant production 4 of 7 positive for production of biosurfactant and emulsification of toluene table 2.
Isolation of biosurfactant producing bacteria soil samples were collected october 30th, 2018 from groundnut oil cake dumping sites at different areas of hyderabad, telangana, india. The present study demonstrates the isolation and characterization of biosurfactant generating bacteria from human breast milk samples. This was evident as no zone of clearance was observed in the blood agar plates. Isolation of biosurfactantproducing marine bacteria and characteristics of selected biosurfactant songklanakarin j. Alternative methodology for isolation of biosurfactantproducing bacteria krepsky, n. Isolation and selection of biosurfactantproducing bacteria. Total 30 bacterial strains were isolated from samples of soil contaminated with petroleum and its products. Enumeration of biosurfactant producing microorganisms from oil contaminated soil in and around bangalore india. The isolation of a new biosurfactant producing bacterium was carried out in soil and water samples collected from jeddah region, ksa. Isolation and screening of biosurfactant producing bacteria from oil contaminated soils in iraq doi.
This study was primarily based on the isolation and identification of biosurfactantproducing bacteria that could utilize palm kernel cake as a new major substrate. Isolation of biosurfactant producing bacteria from garbage soil. These clear zones indicate the presence of biosurfactants producing bacteria. Isolation of potent biosurfactant producing bacteria from oil. Measurements of surface tension, critical micelle dilution and emulsifying activity indicated that the biosurfactant is produced as a primary metabolite. Sep 15, 2014 in this research six different genus of biosurfactant producing bacteria were isolated from persian gulf at bushehr provenance. Isolation and screening of biosurfactantproducing bacteria. Isolation and preliminary screening of lignin degrading microbes. Isolation of biosurfactant producing bacteria from oil reservoirs. The objective of this study was to isolate and screening biosurfactant producing bacteria from petroleum oil contaminated terrestrial samples collected in bangkok, thailand.
Evaluation of screening methods for the isolation of biosurfactant. Introduction biosurfactants are surface active compound that reduce the. Isolation of biosurfactant producing bacteria from petroleum. Characterization and phylogenetic analysis of biosurfactant producing bacteria isolated from palm oil contaminated soils kanokrat saisaard1, atipan saimmai2,3, and suppasil maneerat4 1 faculty of science and technology, suratthani rajabhat university, mueang, surat thani, 84100 thailand.
Nov 11, 2016 hassanshahian m 2014 isolation and characterization of biosurfactant producing bacteria from persian gulf bushehr provenance. In this study, a biosurfactantproducing strain was isolated from a portuguese dairy plant, and the effect of different nitrogen sources on growth and biosurfactant production was evaluated. Marine biosurfactants produced by some marine microorganisms have been paid more attention, particularly for the bioremediation of the sea polluted by crude oil. Haemolysis has been used as an initial selection criterion for the primary isolation of surfactant producing bacteria. Isolation of biosurfactant producers, optimization and properties of. A study on biosurfactant production from marine bacteria. Isolation of biosurfactantproducing marine bacteria and. Production and stability studies of the biosurfactant isolated from.
The objective of the present study is to screen the biosurfactant producing bacteria from soil contaminated with domestic waste water. Isolation and characterization of biosurfactant production under. Isolation and preliminary screening of lignin degrading. Ecofriendly and innovative biocides against biocorrosion. Lignocellulose is the major structural component of woody plants and nonwoody plants such as grasses and represents a major source of renewable organic matter. The biosurfactant producing ability of bacteria was investigated by oil displacement agar plate and emulsification index method. Qualitative analysis of biosurfactant production was identified by performing assays like hemolysis on blood agar, penetration assay and ctab. Isolation and selection of new biosurfactant producing. Isolation and screening of microorganisms from oil contaminated soil samples with biosurfactant producing ability was investigated. Isolation of isolation of biosurfactant producing bacteria from oil reservoirs a tabatabaee 1, m mazaheri assadi 2, a a noohi 1,v a sajadian 3 1faculty of science, research and technology, islamic azad university, iran. Research article isolation and identification of bacillus. Isolation and screening of biosurfactants produced by. Hydrocarboncontaminated sites are the most promising for the isolation of biosurfactant producing microbes, but many strains have also been isolated from undisturbed sites. In this study biosurfactant producing bacteria were isolated from 50 terrestrial samples collected in areas contaminated with petroleum in several districts in bangkok, thailand.
Ten selected biosurfactant producing strains were screened for biosurfactant production by numerous tests. Considering leakage of crude oil from tankers in harbors and other anthropogenic activities, we suspected the occurrence of biosurfactantproducing bacteria at our sampling site ebrahimi et al. Biosurfuctant, bacteria, bacillus subtilis, 16s rrna. Production of biosurfactant by bacillus licheniformis. Screening and characterization of biosurfactant produced by. Easily share your publications and get them in front of issuus. Isolates c1p and nc11p were identified as pseudomonas spp while c1a was identified as azotobacter vinelandii. Biosurfactant produced by none of the isolates had hemolytic properties.
Isolation and screening of biosurfactant producing. Hassanshahian m 2014 isolation and characterization of biosurfactant producing bacteria from persian gulf bushehr provenance. Production of biosurfactants by isolated bacterial strains with different. Identification of biosurfactant producing bacteria. Isolation of isolation of biosurfactant producing bacteria from oil reservoirs a tabatabaee 1, m mazaheri assadi 2, a a noohi 1,v a sajadian 3 1faculty of science, research and technology, islamic azad university, iran 2 biotechnology center of iranian research organization for science and technology, iran 3research institute of petroleum industry, tehran, iran. May 01, 20 issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. Screening and characterization of biosurfactantproducing. The methodology was able to isolate biosurfactantproducing consortia from superficial mangrove sediment contaminated by petroleum hydrocarbons and was recommended for selection of biosurfactant producing bacteria in tropical countries with low financial resources. Two microorganisms showed the highest biosurfactant production when grown on paraffin and glycerol as sole carbon. After screening, candidate biosurfactant producing bacteria were selected for optimization of the physical and.
The present study was focused on isolation of biosurfactant producing bacteria from environmental samples contaminated soil and uncontaminated water and assessing the potential of these isolates. Effects of different ph, salinity and temperature on biosurfactant production were also studied. Correlation between growth and biosurfactant production in. These results are in agreement with the results that reported by other researcher. Thus the present study was carried out to determine the antimicrobial and emulsifying applications of biosurfactant extracted from halobacterium salinarum isolated from sea water. Characterization and phylogenetic analysis of biosurfactant.
The methodology was able to isolate biosurfactant producing consortia from superficial mangrove sediment contaminated by petroleum hydrocarbons and was recommended for selection of biosurfactant producing bacteria in tropical countries with low financial resources. To confirm the ability of isolates to produce biosurfactant, oil displacement test, measurement of surface tension and e24 test were conducted. Yeast extract was found to be an essential component for bacterial growth, while peptone is the most important factor for biosurfactant production. Pdf biosurfactants are microbial amphiphilic compounds which can reduce.
Characterization of biosurfactant produced by probiotic. Screening and isolation of biosurfactant producing bacteria from petroleum contaminated soil pradnya a. Pseudomonas putida, biosurfactant, ennore, optimization. Hemolysis was used as a criterion for the primary isolation of biosurfactant producing bacteria. These isolates were studied for their biosurfactant producing potentials.
Fifty five strains had haemolytic activity, among them twelve strains were good biosurfactant producers by measuring surface tension and emulsification activity. Isolation, screening and characterization of potent biosurfactant producing bacteria from oil contaminated site manish p. Tannery effluents provide an environment for growth of different types of microorganisms. Haemolysis has been used as an initial selection criterion for the primary isolation of surfactantproducing bacteria. Production of biosurfactant produced from used cooking oil. Screening, isolation and characterization of biosurfactant producing. For the isolation of biosurfactant producing bacteria, the sample was collected from automobile workshop in coimbatore. Pdf isolation of biosurfactant producing bacteria from. The screened bacteria were identified by gcfame analysis as bacillus licheniformis. Priyadharshini and latha, 2016 biosurfactant production from bacillus sp. These 37 strains, together with 49 nonhaemolytic ones chosen at random, were studied for surface activity. The biosurfactant producing ability of the isolated strains was investigated by. The bacterial colonies were then observed for the presence of clear zonearound the colonies.
A phylogenetic tree was constructed by combining the results of the present study with a. Only 37 of 492 strains of different origins had haemolytic activity. Isolation and screening of biosurfactant producing bacteria. We isolated and screened potential biosurfactantproducing bacteria. Neighborjoining method was used to calculate the evolutionary.
Three isolates designated c1p, nc11p and c1a were selected for this study on the basis of their characteristics and good growth. The identified bacteria bacillus subtilis and bacillus cereus which were isolated from water and soil respectively were found to possess biosurfactant producing. Pdf isolation and selection of biosurfactantproducing bacteria. Nov 23, 2014 the isolation of a new biosurfactant producing bacterium was carried out in soil and water samples collected from jeddah region, ksa. Research article isolation, screening and characterization. The collected soil samples were kept in sterile bags and transported to experimental laboratory within 2 h before sampling. Materials and methods sample collection for the isolation of biosurfactant producing bacteria, the sample was collected from soil near automobile. Screening concepts for the isolation of biosurfactant. Apart from direct isolation of strains by diluting and plating, enrichment cultures with hydrophobic substrates are very promising for the isolation of biosurfactant producing microbes. Isolation and characterization of biosurfactants producing.
Ten morphologically distinct microbial colonies were isolated and. Therefore, present study focused on the isolation of biosurfactant producing bacteria from. Isolation of biosurfactant producing bacteria from garbage. Isolation of biosurfactant producing bacteria from. May 07, 20 the bacterial colonies were then observed for the presence of clear zonearound the colonies. Isolation of potent biosurfactant producing bacteria from. Surfactants are chemicals that change the properties of water and other fluids. Research projects seeking funding isolation and characterization of biosurfactant producing bacteria from oil polluted environment.
Fortyfive of the isolates were positive for biosurfactant production, representing most of the soils tested. Screening concepts for the isolation of biosurfactant producing microorganisms. We successfully isolated three biosurfactant producers. The sample was taken in sterile polythene bag and was taken to the laboratory and analyzed for the isolation of dye decolorizing bacteria. Isolation and enrichment of biosurfactant producing bacteria two biosurfactant producing bacterial strains were isolated and enriched from diesel oil contaminated soil in dindigul 10. Possibility of occurrence of biosurfactant producers in such effluents collected from local tanneries was investigated in this study.
Isolation of biosurfactant producing marine bacteria and characteristics of selected biosurfactant songklanakarin j. Pdf isolation and selection of biosurfactantproducing. Screening of biosurfactant production was performed using the. Isolation and characterization of biosurfactant producing. Isolation and characterization of biosurfactant production. Ten out of thirty strains showed the biosurfactant activity. Sampling and isolation of bacteria are the basis for screening of biosurfactant producing microbes. Additionally, hydrophobic interaction chromatography and the replica plate technique are also rewarding methods. Screening and optimization of biosurfactant production by. Alternative methodology for isolation of biosurfactant. Abstract biosurfactants are amphiphilic compounds secreted extracellularly by microorganisms. The human breast milk samples were collected aseptically and then cultured in mrs agar media. Soil from automobile workshop and garages in the jaipur city, rajasthan, india. The aim of this study was to introduce palm kernel cake pkc as a novel substrate for biosurfactant production using a potent bacterial strain under liquid state fermentation.
Isolated microorganisms were identified based on the biochemical characterization as. In article 12 walter, christoph syldatk and rudolf hausmann, 2010. Screening and optimization of biosurfactant production by the. Isolation and production of biosurfactant from pseudomonas. Isolation, characterization and identification of bacterial. Screening and isolation of biosurfactant producing bacteria.
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